Typically, experts may need one to two hours to manually formulate a gene targeting strategy that accurately determines the result of gene targeting. During the formulation process, dozens of factors need to be considered and further weighed by their relative importance. Even for the experts who have rich experience, mistakes are inevitable. Additionally, the standards of the best strategy are not objective. Developed by Cyagen, the AlphaKnockout Gene Targeting Expert System provides unbiased strategy design for genetically engineered mouse models by leveraging artificial intelligence (AI) and a robust cloud server that can compute complex biological information algorithms - available as a complimentary resource to researchers and scientists worldwide.

Diverse Methods
Cyagen provides conventional and conditional knockout mouse models strategies using CRISPR/Cas or ES cell (homologous recombination) gene targeting techniques.
 
 

Fast
Complete reports will be provided by searching for the knockout gene target on the above search bar. The system offers up to three reports prioritized based on bioinformatics algorithms as fast as 10 seconds, with the first one being the most optimal solution.

Intelligent
By unitizing the robust Tianhe-2 supercomputer, AlphaKnockout Gene Targeting Expert System performs large-scale intensive computing to apply AI for optimized and unbiased gene targeting strategies of genetically engineered mice.

Complimentary
Cyagen promises that the AlphaKnockout Gene Targeting Expert System is permanently free and open for use.                   
 
 

After researchers obtain clinical samples, next-generation sequencing (NGS) and genome-wide association studies (GWAS) may be used to identify many single nucleotide polymorphisms (SNPs) and candidate loci. However, to verify the correlation between these loci and clinical results, in vivo experiments are required. Point mutation mice can work as an excellent research tool for more detailed studies at this stage.

Search by gene target on Cyagen’s Point Mutation Strategy Design System for one-click access to point mutation mouse models, relevant mutation site research literature, clinical disease characteristics, and custom gene targeting strategy reports. Together, these tools can directly serve as valuable information and materials for clinical research, drug development, and molecular diagnostics in precision medicine. Beyond serving as a practical tool, it is also a system designed to inspire researchers seeking to understand the point mutant mouse model construction process via source-based data analysis.

Massive Mutant Loci Database
More than 200,000 point mutation strategies are available: just query by gene name and select the desired gene mutation site with one click.

Customizable Strategies
Our robust system offers DIY strategy customization for your desired point mutation models.

Powerful Function
Apart from displaying basic mutation site information, the system also compiles mutation site-related research literature, clinical disease characteristics, and more.
 

User-friendly
Get one-click access to information about mouse models across mutation sites and receive model construction guidance, compiling numerous resources to make the user experience more convenient.

MicroRNAs (miRNAs) play an essential role in cell differentiation, biological development, and the development of disease. Alongside the in-depth studies of the relationship between microRNAs and diseases with the use of high-throughput technologies such as microRNA microarray chips , the collective understanding of the network of gene expression and regulation in higher eukaryotes has been taken to a new level. MicroRNAs may become new biological markers for disease diagnosis, even serving as drug targets or as a target mimic for new molecule drug development, which may provide a new approach of treatment for human diseases. Cyagen’s bioinformatics team has integrated information from primary microRNA databases worldwide to develop a microRNA knockout mouse database with powerful bioinformatics functions that uses AI to select and design the optimal models using all available data.

Fast
Access complete reports by searching for the gene name in the above search bar.
 
 

Intelligent
Access microRNA details and gene knockout modeling guidance with one click, compiling numerous resources to make the user experience more convenient.

Complimentary
Cyagen promises that the microRNA Knockout Strategy Design System is permanently free and open for use.
 

Through the artificial intelligence (AI)-driven combination of AlphaKnockout Gene Editing Expert System and CRISPR/Cas technology, Cyagen has developed the Smart-CRISPR^™ Cell Gene Editing System. Smart-CRISPR^™ is more efficient in gene cleavage than CRISPR/Cas technology alone and can easily accomplish multiple knockout strategies in cell lines - such as frameshift mutation, fragment knockout, and multiple gene knockout - to provide scientific solutions to problems such as protein-positive residues.

Intelligent
Through AI-driven AlphaKnockout Gene Targeting Expert System and the powerful Tianhe-2 for large-scale computing, Smart-CRISPR^™ Cell Gene Editing System optimizes the process of formulating genetically engineered cell lines strategies with gene-editing technology.

Fast
Search for the knockout gene target on the above search bar and the system will present a complete report based on available bioinformatics data and processing algorithm in as fast as 10 seconds.

Diverse Methods
Multiple knockout strategies such as frameshift mutation, fragment knockout, and multiple knockouts can be achieved easily.

Complimentary
Cyagen promises that the Smart-CRISPR^™ Cell Gene Editing System is permanently free and open for use.